Fluorescent Quantitative Detection System Real-Time PCR System
| Customization: | Available |
|---|---|
| Capacity: | 98 Well |
| Channels: | 6 Channels Fluorescence Detection |
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Basic Info.
- Model NO.
- PCR SYSTEM
- Prevent Reagent Evaporation
- Yes
- Fluorescence
- Yes
- Sample Test Repeatability
- CV<3%
- Fluorescence Linearity
- R≧0.99
- Transport Package
- Carton Box
- Specification
- 38*38*26
- Trademark
- SAH
- Origin
- China
- Production Capacity
- 5000000
Product Description
Real Time 48-well 96 Well Real time PCR Laboratory Thermal Cycler Gradient gene amplification instrument
Quantitative real time PCR detection system Machine
Whole block scanning and formulated line scanning mode
98 well double colour scanning takes only 5.5s.
6 channels fluorescence detection
No cross talk between different channels unique bottom detection compatible to reaction volume down to 5micro liter
New automatic hot lid
Automatic open and close which is prevent reagent evaporation
LED excitation light source with super long service life
Features
-
Advanced optical system including full-spectrum high-power LED and high-sensitive PMT top scanning achieves higher sensitivity you need in researches and experiments.
-
High-precision temperature control over the block with a resolution of 0.1 ºC offers reliable and consistent qPCR assay data.
-
Gradient temperature range is 30-100ºC and the temperature difference between adjacent blocks can be high up to 40ºC for assay optimization.
-
Sample tray automatically moves in/out, enabling integration with automated workstations/plate handlers for unmanned detection.
-
6 fluorescence channels without cross talk and 96-well high-throughput process at a single run meet a variety of qPCR assay demands.
-
The 10.1-inch large touch screen facilitates flexible programming and efficient running of PCR protocols.
-
The intuitive built-in software and PC software for improved user's experience allow you to run and analyze qPCR data from personal computer
-
Two temperature control modes enable you to control either block or tube temperatures.
Multiple Applications
The Real-Time Fluorescent Quantitative PCR System uses fluorescence real-time detection method to analyze the amplification of PCR template. It is suitable for human genome engineering, forensic medicine, oncology, population biology, paleontology, zoology, botany and other research fields.
This PCR detection system can be an in vitro diagnostic medical device, which is suitable for polymerase chain reaction fluorescence quantitative detection in the field of clinical diagnosis of viruses, tumors, genetic diseases, etc.
PCR machine is also known as thermal cyclers or DNA amplifiers, or thermocyclers. PCR machines amplify small segments of DNA or RNA chosen from the genome with a primer. It is inexpensive and a very effective tool.Real-time PCR, also known as quantitative PCR or qPCR, is a technique for monitoring and quantifying PCR results in real-time by labeling DNA molecules with fluorescent dye.
PCR Applications
PCR can investigate differences in gene transcription among various cell types, tissues, and species at a particular time period.
- Reverse transcription is used to create cDNA by isolating RNA from samples of interest.
- The quantity of cDNA produced by PCR can then be used to calculate the original RNA levels for a particular gene.
- Sequence differences in alleles of certain cells or organisms can be determined.
- Genotyping of transgenic organisms facilitates the amplification of the mutation or a transgenic part.
- PCR cloning makes it possible to breed new strains of bacteria with altered genetic makeup via the insertion of amplified dsDNA fragments into the vectors such as gDNA, cDNA, and plasmid DNA.
- Cloning assists in introducing point mutations using site-directed mutagenesis which itself employs the recombinant PCR method.
- It also helps to create novel gene fusions.
- Sequencing accompanies the amplification of template DNA, their purification, and processing through a sequencing step.
- PCR is also employed in next-generation sequencing (NGS) during the library preparation phase to quantify DNA samples and tag them with sequencing adaptors for multiplexing.
- Medical applications include genetic changes linked to disease to identifying infectious organisms. Prenatal genetic testing employs PCR to detect chromosomal abnormalities and genetic mutations in the pregnancy, providing expecting parents with crucial information about the likelihood that their children will have a particular genetic illness.
- It can also be employed as a preimplantation genetic diagnosis technique to screen embryos for in vitro fertilization (IVF).
- PCR can be useful for forensic investigations to identify the sources of samples and paternity testing.
- It is used in molecular archaeology to amplify DNA from artifacts.
- Pathogens can be found using PCR, not just in patient samples but also in matrices like food and water. This is important for both diagnosing and preventing infectious diseases.
PCR involves three main steps repeated in cycles:
1.Denaturation
The mixture is heated to 94°C for 0.5 to 2 minutes.
This heat breaks the hydrogen bonds between DNA strands, turning them into single strands.
The single strands serve as templates for creating new DNA strands.
2.Annealing
The temperature is lowered to 54-60°C for 20-40 seconds.
Primers, which are short DNA or RNA sequences, bind to the complementary sequences on the single-stranded DNA.
Each separated strand gets a forward and reverse primer.
3.Elongation
The temperature is raised to 72-80°C.
The taq polymerase enzyme adds DNA bases to the 3' end of the primer, extending the DNA strand in the 5' to 3' direction.
This enzyme can withstand high temperatures and works quickly, adding about 1000 bases per minute.
These three steps are repeated 20-40 times to rapidly amplify the target DNA sequences.
Specifications
|
Throughput |
96 well (12X8) |
|
Applicable consumables |
PCR single tube and strip of 0.2 ml, 96-well half skirted / no skirted plate PCR single tube and strip of 0.1 ml, 96-well no skirted plate |
|
Dynamic range |
1-1010copies |
|
Detection location |
Top detection |
|
Excitation light wavelength |
400-800nm |
|
Detection wavelength |
500-800nm |
|
Fluorescence channels |
6 channels |
|
Fluorochrome / dye |
Channel 1: FAM/SYBR Green I Channel 2: JOE/HEX/TETA/IC Channel 3: NED/TAMRA/Cy3 Channel 4: ROX/Texas Red Channel 5: Cy5 Channel 6: Cy5.5 |
|
Excitation light source |
full-spectrum LED |
|
Detector |
PMT |
|
Block temperature range |
4-105°C |
|
Block temperature accuracy |
≤±0.1ºC |
|
Block temperature precision |
≤±0.1ºC |
|
Block temperature uniformity |
≤±0.1ºC |
|
Max. heating & cooling rate of block |
≥6ºC/S |
|
Gradient temperature difference |
1-40°C |
|
Block temperature control mode |
Block mode, Tube mode |
|
Sample volume range |
5-100 µL |
|
Heated lid temperature range |
30°C-110°C (default 105°C) |
|
Fluorescence intensity |
CV ≤3% |
|
Detection repeatability |
Yes |
|
Touch screen |
10.1-inch touch screen |
|
Scan mode |
Full plate scan or specified line scan |
|
Software functions |
Absolute quantification, Relative quantification, Melting curve, SNP genotyping, HRM, Quick run, etc. |
|
Operation system |
PC software (Windows 8 or higher), Built-in software |
|
Ports |
USB Type-A port x 2, USB Type-B port, RJ45 port |
|
Power-off protection function |
YES. Data can be restored after power-on |
|
Sample tray control mode |
Sample tray automatically moves in/out, enabling integration with automated workstations/plate handlers. |
|
Dimension (WxDxH) |
320mm x 525mm x 420mm |
|
Net weight |
≤23kg |
| Thermal Cycling Technology | Peltier Module |
| Memory | 8GB |
A real-time PCR system is a powerful laboratory instrument that enables researchers to monitor and quantify DNA or RNA amplification in real-time. This technology relies on the use of fluorescent markers that emit signals during each amplification cycle, allowing for the accurate determination of the starting amount of genetic material. It can also simultaneously detect and quantify multiple target sequences in a single reaction, saving time and resources. The high sensitivity, precision, and versatility of real-time PCR make it an essential tool across an array of scientific domains such as genetics research, clinical diagnostics, microbiology, and environmental monitoring. In practice, it furnishes researchers with an unparalleled opportunity to delve into the intricacies of gene expression, diagnose diseases, and undertake a broad spectrum of molecular biology investigations with unwavering accuracy and operational efficiency.
The LineGene 9600+ Real-Time System combines high-performance Peltier technology with an extremely sensitive detection system. Extensive software ensures flexibility for all qPCR applications.
The experimental setup, control and data analysis are carried out either on a Windows PC or a tablet.
| Detected Fluorescence |
F1:FAMSYBR Green 1; F2:VICHEXTETJOECY3TAMRA; F3:ROXTEXAS-RAD F4:CY5Quasar-670; F5:CY5.5Quasar-705; |
| Block Temp.Range |
4 ~105 ºC ( Minimun Increment : 0.1 ºC ) SOAK Low Temp.Consercation Function |
| Temp.Control Mode | BLOCK/Tube Simulation Mode (Automatic Control based On Sample Volume ) |
| Gradient Temp.Range | 1 ~36 ºC |
| Hot-lid Temp.Range | 30 ~110 ºC (Adjustable default 105 ºC Automatic Hot-lid ) |
| PC Configuration | Memory:512M Hard Disk:10GB CPU:Pentium® 4 Virtual Memory:≥1000MB |
| Operation System |
Microsoft : Windows XP/Windows vista/Windows 7/Windows 8 Software : Excel2000/2002/2003/2007/2012 |
| Power Supply | 100-240V ~ 50/60Hz 600W |
| Dimension ( L×W×H ) | 410mm×386mm×352mm |
| socket | USB Adapter |
| Authentication | Ferrotec Pelitier / CE/Met / RoHS2 / PICC product quality liability insurance |
Equipment category: category l (equipment category l with protective grounding device and
structure category ll)
Insulation category: reinforced insulation
Power supply: 100-240V~,50/60Hz
Input power: 600W
Operation: continuous operation
Pollution class:l
Protective class:lP20
Electromagnetic compatibility: Class A
Applicable region: areas under 2000m without high temperature or high humidity
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