Real-Time Fluorescent Quantitative PCR System 96 Wells

• Customization: Available
• Capacity: 98 Well
• Channels: 6 Channels Fluorescence Detection
• Model NO.: PCR System
• Prevent Reagent Evaporation: Yes
• Fluorescence: Yes
• Sample Test Repeatability: CV<3%
• Fluorescence Linearity: R≧0.99
• Transport Package: Carton Box
• Specification: 38*38*26
• Trademark: SAH
• Origin: China
• Production Capacity: 5000000

Product Description

Fluorescent Quantitative PCR Detection System
Feature:
♦ Six independent temperature control
♦ Thermal compensation technology of temperature module
♦ No edge effect of temperature and optics have
♦ Maintenance-free long - life continuous spectral excitation source
♦ Synchronous and fast acquisition of low temperature CCD 0.15s in cold state
♦ Relative quantitative high sensitivity 1.5 times effective discrimination
♦ Flexible, open and user-friendly software platform
♦ Open reagent consumable platform

Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.
 

Application:
♦ Basic materials research
♦ Pathogen detection
♦ Public health safety surveillance
♦ Meat products adulterated
♦ Transgenic testing
♦ Food safety inspection
♦ Drug development and rational drug use
♦ Precise tumor treatment
♦ Gene expression
♦ Genetic screening
♦ Genotyping
♦ Stem cell research

Temperature control module
On the basis of the six independent temperature control technology, using the enclosed liquid heat transfer efficiency, combined with environmental scanning monitoring regulation auxiliary infrared with heating and intelligent variable frequency system voltage, current, ensures the module heating speed, effectively prevents temperature overshoot no edge effect and the evaporation temperature, not only saves your precious time to amplify the required temperature uniformity and the repeatability of the results achieved perfection, no matter what kind of test mode, application, it can get good data uniformity and repeatability.
 

Optical module

Optical module

On behalf of the world's leading optical transmission and collection technology, use optical fiber after the excitation light source for reaction system and the emission of light from the reaction system after excitation, the energy attenuation without conduction to each reaction holes and cold CCD, from physical properties to ensure consistency and authenticity of the excitation and detection, and greatly improve the detection sensitivity, can make your low effective detection and distinguish easily copy sample. The channel mismatch function is added to extend the application field of qPCR to the protein level and provide a new way for the construction of multiple systems of diagnostic reagents.

 

Channel	Excitation wavelength (nm)	Emission wavelength (nm)	Examples of fluorescent dyes
1 Blue	460-480	512-528	FAM/SYBR Green I/Eva Green…
2 Green	515-535	562~578	VIC/JOE/HEX/TET…
3 Yellow	560~580	612~628	ROX/Texas Red …
4 Red	610-630	662~678	Cy5…
5 Red	660-680	702~718	Cy5.5/Quasar705…

Multiple Applications

The Real-Time Fluorescent Quantitative PCR System uses fluorescence real-time detection method to analyze the amplification of PCR template. It is suitable for human genome engineering, forensic medicine, oncology, population biology, paleontology, zoology, botany and other research fields.

This PCR detection system can be an in vitro diagnostic medical device, which is suitable for polymerase chain reaction fluorescence quantitative detection in the field of clinical diagnosis of viruses, tumors, genetic diseases, etc.

PCR machine is also known as thermal cyclers or DNA amplifiers, or thermocyclers. PCR machines amplify small segments of DNA or RNA chosen from the genome with a primer. It is inexpensive and a very effective tool.
Real-time PCR, also known as quantitative PCR or qPCR, is a technique for monitoring and quantifying PCR results in real-time by labeling DNA molecules with fluorescent dye.

Channel	Excitation wavelength (nm)	Emission wavelength (nm)	Examples of fluorescent dyes
1 Blue	460-480	512-528	FAM/SYBR Green I/Eva Green…
2 Green	515-535	562~578	VIC/JOE/HEX/TET…
3 Yellow	560~580	612~628	ROX/Texas Red …
4 Red	610-630	662~678	Cy5…
5 Red	660-680	702~718	Cy5.5/Quasar705…

♦ Channel combination: 6-color excitation light filter and 6-color detection light filter can detect≥21 different fluorescence spectra, channels are completely open, supporting third-party reagent optimization Support FRET application.

♦ Multichannel static fluorescence function: analysis of starting template and end product.

♦ Filter can be customized according to customer need

 

 

Software platform

Real-time monitoring, automatic discrimination and calculation of positive and negative results, automatic establishment of standard curve, absolute/relative quantitative, multiple quantitative, fusion curve, gene mutation, quality control graphic analysis, fluorescence correction, PCR amplification efficiency, high-resolution fusion curve (optional match), etc.

PCR Applications
PCR can investigate differences in gene transcription among various cell types, tissues, and species at a particular time period.

• Reverse transcription is used to create cDNA by isolating RNA  from samples of interest.
• The quantity of cDNA produced by PCR can then be used to calculate the original RNA levels for a particular gene.
• Sequence differences in alleles of certain cells or organisms can be determined.
• Genotyping of transgenic organisms facilitates the amplification of the mutation or a transgenic part.
• PCR cloning makes it possible to breed new strains of bacteria with altered genetic makeup via the insertion of amplified dsDNA fragments into the vectors such as gDNA, cDNA, and plasmid DNA.
• Cloning assists in introducing point mutations using site-directed mutagenesis which itself employs the recombinant PCR method.
• It also helps to create novel gene fusions.
• Sequencing accompanies the amplification of template DNA, their purification, and processing through a sequencing step.
• PCR is also employed in next-generation sequencing (NGS) during the library preparation phase to quantify DNA samples and tag them with sequencing adaptors for multiplexing.
• Medical applications include genetic changes linked to disease to identifying infectious organisms. Prenatal genetic testing employs PCR to detect chromosomal abnormalities and genetic mutations in the pregnancy, providing expecting parents with crucial information about the likelihood that their children will have a particular genetic illness.
• It can also be employed as a preimplantation genetic diagnosis technique to screen embryos for in vitro fertilization (IVF).
• PCR can be useful for forensic investigations to identify the sources of samples and paternity testing.
• It is used in molecular archaeology to amplify DNA from artifacts.
• Pathogens can be found using PCR, not just in patient samples but also in matrices like food and water. This is important for both diagnosing and preventing infectious diseases.

The PCR Machine promotes polymerase chain reaction (PCR) allowing vitro amplification of DNA fragments as fast cloning, DNA sample modification, detecting pathogenic microorganisms and accurate genotyping, detection of mutation, malignancy transformation or tissue typing, analysis of the archaeological specimen, forensic applications, paternity testing, mapping hereditary traits, and investigating gene expression.  

PCR steps
PCR involves three main steps repeated in cycles:
1.Denaturation
The mixture is heated to 94°C for 0.5 to 2 minutes.
This heat breaks the hydrogen bonds between DNA strands, turning them into single strands.
The single strands serve as templates for creating new DNA strands.
2.Annealing
The temperature is lowered to 54-60°C for 20-40 seconds.
Primers, which are short DNA or RNA sequences, bind to the complementary sequences on the single-stranded DNA.
Each separated strand gets a forward and reverse primer.
3.Elongation
The temperature is raised to 72-80°C.
The taq polymerase enzyme adds DNA bases to the 3' end of the primer, extending the DNA strand in the 5' to 3' direction.
This enzyme can withstand high temperatures and works quickly, adding about 1000 bases per minute.
These three steps are repeated 20-40 times to rapidly amplify the target DNA sequences.

Specifications

Product Name	Fluorescence Quantitative PCR Detection System
Model	MA-6000
Sample Capacity	96x0.2ml PCR plate, 12x8-strip tubes, 96*0.2ml single tube
Reaction System	6~125μl
Dynamics Range	1-1010 copies
Channel	5
Emission Light	Full-wavelength maintenance-free halogen tungsten lamp
Detector	-20ºC CCD
Detection Path	F1	F2	F3	F4	F5
Suitable Probe/Dye	FAM/SYBR Green/Eva Green/LC Green/Fluorescein	VIC/HEX/TET/Cy3/JOE/Alex a555	ROX/Cy3.5/Texas Red	Cy5/LC Red640	Cy5.5/LC Red705
Excitation Wavelength	380~780nm
Detection Wavelength	380~780nm
Fluorescence Detection Repeatability	CV≤3%
Fluorescence Detection Precision	CV≤ 5%
Sample Detection Repeatability	CV≤3%
Fluorescence Detection Linearity	r≥0.980
Confidence	It can effectively distinguish 5000 and 1000copies with99.8% confidence degree
Module Temp. Range	4-100ºC(resolution:0.1ºC)
Heating Speed	5.0ºC/s(Max), 3.5ºC/ s(Avg)
Cooling Speed	5.0ºC/s(Max), 3.2ºC/ s(Avg)
Temp. Accuracy	±0.1ºC
Temp. Uniformity	≤ ±0.25ºC
Number of Temp. Control Areas	6
Gradient Temp. Range	1-32ºC
Hot-Lid Temp. Range	104ºC, Automatic Hot-lid
Special Function	Absolute quantification, Relative Quantification, Genotyping, Amplification efficiency calculation, Melting curve.
Operation System	Windows 7 64-bit or higher
Power Supply	100~240V,50~60Hz
Dimension(L*W*H) mm	600*390 *320
Port Method	USB Port
Packing Size(L*W*H) Mm	850*600 *650
Gross Weight	51

A real-time PCR system is a powerful laboratory instrument that enables researchers to monitor and quantify DNA or RNA amplification in real-time. This technology relies on the use of fluorescent markers that emit signals during each amplification cycle, allowing for the accurate determination of the starting amount of genetic material. It can also simultaneously detect and quantify multiple target sequences in a single reaction, saving time and resources. The high sensitivity, precision, and versatility of real-time PCR make it an essential tool across an array of scientific domains such as genetics research, clinical diagnostics, microbiology, and environmental monitoring. In practice, it furnishes researchers with an unparalleled opportunity to delve into the intricacies of gene expression, diagnose diseases, and undertake a broad spectrum of molecular biology investigations with unwavering accuracy and operational efficiency.

Product information LineGene 9600+ Real-Time PCR System

High-Sensitivity Multiplex Optics
The LineGene 9600+ Real-Time System combines high-performance Peltier technology with an extremely sensitive detection system. Extensive software ensures flexibility for all qPCR applications.
The experimental setup, control and data analysis are carried out either on a Windows PC or a tablet.

Software InterfaceThe LineGene 9600 Plus software includes Abso-lute Quantification, Relative Quantification, SNPAnalysis, HRM Analysis function modules. Withthe preset programs, the user can set up experi-ments simply and fast. The recently opened filesmake it easy to find previous experiments.

Working principle 

Working Principle: The heating and cooling of PCR blockduring PCR processes is done by an inbuilt Ferrotec peltiertechnology. The LED excitation source in the system pairedwith advanced fiber optic transmission technology makes thephotoelectric detection system very sensitive and reliable.The florescent emission is detected by Photomultiplier Tube(PMT).The Stepper motor with switchable filters is driven toeach and every sample by a synchronous belt for sys-temic scanning of sample from different wells.Block temperature and fluorescence detec-tion are controlled by microcomputer.The upper computer controls ex-periment operation and dataanalysis.
 

 

Detected Fluorescence	F1:FAMSYBR Green 1; F2:VICHEXTETJOECY3TAMRA; F3:ROXTEXAS-RAD F4:CY5Quasar-670; F5:CY5.5Quasar-705;
Block Temp.Range	4 ~105 ºC ( Minimun Increment  :  0.1 ºC ) SOAK Low Temp.Consercation Function
Temp.Control Mode	BLOCK/Tube Simulation Mode (Automatic Control based On Sample Volume )
Gradient Temp.Range	1 ~36 ºC
Hot-lid Temp.Range	30 ~110 ºC (Adjustable default 105 ºC Automatic Hot-lid )
PC Configuration	Memory:512M     Hard Disk:10GB    CPU:Pentium® 4    Virtual Memory:≥1000MB
Operation System	Microsoft  :  Windows XP/Windows vista/Windows 7/Windows 8 Software  :  Excel2000/2002/2003/2007/2012
Power Supply	100-240V ~ 50/60Hz   600W
Dimension ( L×W×H )	410mm×386mm×352mm
socket	USB Adapter
Authentication	Ferrotec Pelitier / CE/Met / RoHS2 / PICC product quality liability insurance

 

 

Basic structure of instrument
Equipment category: category l (equipment category l with protective grounding device and
structure category ll)
Insulation category: reinforced insulation
Power supply: 100-240V~,50/60Hz
Input power: 600W
Operation: continuous operation
Pollution class:l
Protective class:lP20
Electromagnetic compatibility: Class A
Applicable region: areas under 2000m without high temperature or high humidity

 

 

 

 

 

 

 

 

 

Production workshop

The production of the Automatic Pathological Tissue Microtome machine machine introduce
1.Start by the blanking process for the metal part

Normally the house of the Slide Stainer medical was made by the steel or stainless steel of type as 316
The part was beginning as the blanking
1.Laser blanking machine capacity :8000*2000*10mm
2.We had about 15 pcs of the laser cutting machine in blanking workshop ,name from No1 to No 15,
3.6S fully carry out in our workshop according to the iso13485,
4.All of the raw material can be Traceability

2.Bending process for Laboratory equipment Tissue Microtome machine by the press break bending machine

 

3.Progressive die metal stamping workshop for Slide Stainer

 

4.Welding workshop for the Tissue Microtome machine

 

5.Plastic injection workshop for the Benchtop Steam Automatic Slide Stainer machine

Plastic part was one part of the Horizontal Universal Laboratory equipment Microtome machine
We had about 10 sets of the plastic injection work for the Laboratory equipment Microtome machine
1.The capacity for plastic injection machine was :1000*850*750mm
2.The max ton for plastic injection machine was 1000T

6.The spare part Warehouse for the benchtop Stainless Steel Multifunction Steam Sterilization machine

The 6S was fully carry out in our warehouse with the ERP system
1.We need to know how many part in our warehouse
2.We need to anticipate the part when to full fill in for all the Automatic Slide Stainer with Laboratory equipment Microtome machine

7.CNC machining for the benchtop Microtome machine

 

8.Universal Digital Ultrasonic Semi Automatic Microtome stamping ,welding ,rolling Production process on site,the container of the Ultrasonic Semi Automatic Microtome Machine need the vacuum and seal can bear though high pressure

9.Electric part assemble to the Multifunction Steam Sterilization Machine workshop,the plc or the other computer control part connect to the machine before move to the next production procss.

10.Test workshop for the Multifunction Steam Sterilization Machine

Customer visit

Customer review our Fully Automated Tissue Steiner workshop,We were welcome all of our customer to visit our company to review and test the Fully Automated Tissue Steiner for Organisms, Medical, Chemistry,Our business vision was :long term business ,win-win for the future

 

 

Exhibition

We take part into the MedtecChina Canton Fair (The China Import and Export Fair) exhibition every year ,hope one day we can meet each on the fair

Our vision

The supplier and the customer was togeher for the future ,both parties should be in win-win situation ,so we were looking forwarder to hold hand by hand for bright future.