Tissue Processing: Principle, Steps Of Tissue Processing, All machine equipment apply for the tissue processing
Principle of Tissue Processing
- The technique of getting fixed tissue into paraffin is called tissue processing
- Tissue processing is designed to remove all extractable water from the tissue, replacing it with a support medium that provides sufficient rigidity to enable sectioning of the tissue without damage or distortion
Fick's Law
- To equalize concentrations inside and outside blocks of tissue this depends on Fick's Law:
- the rate of solution diffusion through tissues is proportional to the concentration gradient (the difference between the concentrations of the fluids inside and outside the tissue) as a multiple of temperature dependant constants for specific substances.
Steps Of Processing:1.Dehydration; 2.Clearing;3.Embedding;4.
Vacuum Spin Tissue Processor- Vacuum Spin Tissue Processor
1. DEHYDRATION
• The first stage of processing is the removal of free unbound water and aqueous fixatives from the tissue components.
• Many dehydrating reagents are hydrophilic and interact with the water molecules in the tissue by hydrogen bonding.While Other reagents affect dehydration by repeated dilution of the aqueous tissue fluids.
• Excessive dehydration may cause the tissue to become hard, brittle and shrunken.Incomplete dehydration will impact the penetration of the clearing reagents into the tissue leaving the specimen soft and non-receptive to infiltration.
2. Clearing
Clearing is the transition/intermediate step between dehydration and embedding.Consists of removal of the dehydrant with a substance that will be miscible with both the embedding medium paraffin and dehydrating agent.
Laboratory Benchtop 12 stations Automatic fully Pathology Histology baking Linear Vacuum Spin Tissue Processor
1.All 12 station time can be individually programmed using a pin-up timer
2.In-built safety device to protect the tissues in case of power failure
Imported motor and sturdy gear assembly ensures smooth and silent shifting of tissue baskets
3.Thermostatically controlled wax baths with temperature range of ambient to 80 deg C.
4.Continuous rotation of tissue baskets ensures better tissue processing
3.Specification
- Short Desc :Spin Tissue Processor
- Tanks: 12 stations , 10 reagent container ,
- 2 wax paraffin container
- Option : two basket loading, 3 Wax baths"
- Capcity: 2.3 L for reagent container ,
- 1.8 L for wax parafin container" "
- Tissue Baskets : Size :Φ 120X100mm
- Number: 1 (2 optinal ), Capacity : max 100 cassettes"
- Temperatuer :45 -85
- Programs: 8 sets
- Vaccuum device pressure : max 0.05 Mpa(approx 0.5 bar)
- Net weight : 80 kg
- Instrument dimension : Φ780 mmX height : 570mm-690mm Nominal voltage : 220 V AC±10%
- Nominal frequency : 50/60Hz Power : 800W
4.Feature
- Microcomputer controlling to ensure tissue basket relocating precious
- Electricity protection and obstacle protection functions
- Agitation dehydrate mode 10 achieve good dehydrate effect
- Large LCD screen monitoring and showing every step of processing
- Timning : delay timing max 99 days 99 hours 59 mins
- Jar processing time : Max 99 hours 59 mins timeing increment : 1 min"
- Constant temperature controlling system adopted on the paraffin jar to ensure high precision of temperature controllong .
- Internal surface that provide the good condition for tissue protection
- There are 8 programs selectable
- Battery backup for 8 hours except the paraffin jar
- Addopted with electric lock and passward for specific person use
Tissue embedding center and cooling plate histology tissue embedding center for lab
Embedding tissues in paraffin wax .Requirements for embedding are as follows:
1.Clean, filtered paraffin wax held at 2-4°C above its melting point.
2.Paraffin dispenser
3.Cold plate to rapidly cool the wax.
4.Moulds in which to embed the tissues.
5.Paraffin wax is dispense automatically from a nozzle into a suitably sized mold.
6.The tissue is oriented in the mold, a cassette is attached.
7.The mold is placed on a small cooling area to allow the paraffin
Tissue Embedding Center & Cooling Plate
* Microprocessor Control for temperature,made in the USA
* The Embedding Module and Cryo Module can be assembled freely
* Flexible heating mechanism, Dual-protection; Safe, Reliable and Energy-Saving
* Fully programmable computer controls allow automatic system start and stop anytime within a week
* Automatic Memory and Restoration: After start, all preset temperature data are automatically stored
* Paraffin Chamber, Paraffin Dispenser, Left and Right Thermal Storage Compartments, and Heating Plate (working area) are
individually controlledOptional:
* Cooling Plate Working Temp.: -20ºC~RT Working Size: 320*300mm
Product Features:
- Temperature control adopts advanced American microprocessor controller, PID five-way independent temperature control, no mutual influence
- Simple panel design, function blue LED indication, temperature color-changing LED indication, working status is clear at a glance
- International advanced flexible heating concept, overcomes the disadvantage of large temperature difference of traditional heating, fast heating and high precision
- Double overheat protection, avoids the shrinkage of paraffin exceeding 70ºC and damaging tissue specimens
- With memory and automatic recovery function, the preset temperature is automatically saved after operation
- The front and rear double switch design of the freezing table is more convenient and safer to use
- The imported valve adjusts the flow of the wax nozzle, which is suitable for various tissue embedding treatments
- Large area granite workbench, beautiful and atmospheric, convenient for paraffin cleaning
Paraffin Chamber Capacity
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3L
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Temp.Range of
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Forceps Wells
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55~70ºC
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Paraffin-melting Chamber
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Thermal Storage Compartments
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Heated Working Areas
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Paraffin Dispenser
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Temp. Precision
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±1%
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Material of Work Table
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Granite, easy to clean
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Paraffin Flow Control
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Finger touch plate and optional foot pedal
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Power Supply
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AC110/220V±10%, 50/60Hz
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Consumption
|
650W
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External Size(W*D*H)
|
525*550*385mm
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Package Size(W*D*H)
|
630*670*520mm
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Gross Weight
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41kg
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Optional Accessory
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BK-CPI Cooling Plate
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Package Size of BK-CPI (W*D*H)
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700*450*530mm
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Gross Weight of BK-CPI
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37kg
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Medical Laboratory Sliding Microtome Tissue Pathological Microtome
Features:
- Streamlined molded housing results in attractive appearance and is easy to clean.
- Full-automatic sectioning with adjustable speed and three sectioning modes: continuous, step and single.
- LED display
- Electronic trim function makes it easy to switch from trim and section mode
- Specimen retraction mechanism protects specimen from unintended blade damage
- Section counting function counts total section pieces and total thickness.
- Unit contains a precise micro motion specimen feeding system which uses a micro computer, optical signal and stepper motor to control specimen feeding resulting in more precise specimen feeding.
- Internationally accepted slide mechanism and groove ensures the precision of specimen movement while making the unit free from lubrication and maintenance requirements.
- Unit contains a handwheel force balancing system for adjusting the balancing force during sectioning, making rotation even and smooth;
- Two safety locking mechanisms on the handwheel; one locks on the top; the other locks at any
position
- The disposable blade can move horizontally to avoid the dangers of unintended contact with the blade. Every blade cuts specimen into three equal parts, which ensures the entire use of blade edge
- The waste tray collects sectioning debris are easily removed for cleaning
- Cassette clamp and C clamp are optional
- Specimen orientation on X/Y axes 8°
- Alarm to warn of forward or backwards limitation.
- Self-diagnostic prompt.
- Emergency stop and foot switch (optional)
1.The specimen retraction protects the specimen and make the section more precise.
2.The red protection guard is to cover the cutting edge when the blade is inserted into the blade holder.
3.Changeable specimen clamp system.
4.For the optimal position of the blade for sectioning the angle of the cutting facet of the blade can be adjusted by moving the blade holder.
Collects the sectioning wastage and can be dismounted easily for cleaning purposes.
5.Right operation hand-wheel can be locked at any position, providing maximum safety and conve
Main Technical Data:
- Section thickness range:0.5μm~100μm(3~5um is the best slicing effect)
- Section thickness setting:0.5~5μm,in 0.5μm increment
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- 5~20μm,in 1μm increments
- 20~60μm,in 5μm increments
- 60~100μm,in 10μm increments
- Trimming thickness: 1~600μm
- Trimming thickness setting:1~10μm,in 1μm increment ;
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- 10~20μm,in 2μm increments
- 20~50μm,in 5μm increments;
- 50~100μm,in 10μm increments;
- 100~600μm,in 50μm increments;
- Specimen retraction: 10~150μmμm(Can be closed),in 1μm increments ;
- Specimen vertical stroke:60mm
- Specimen horizontally stroke:25mm
- Max specimen size with C clamp (H×W):55mm*45mm
- Dimensions (W×D×H):570*510*490mm
- Net Weight:34.6kg
Tissue Water Bath
- water bath is used for floating out tissue ribbons after sectioning.
- The trailing end of the ribbon making contact with the water first
- The temperature of the water in the bath should be 10°C below the melting point of the paraffin.
- Alcohol or a small drop of detergent may be added to the water allowing the section to flatten out with greater ease
- 30 seconds are long enough for a ribbon to flatten
Features:
Quick heating, long lifespan and energy saving, due to the use of a new-type heating element;
Both actual and preset temperatures are displayed;
Automatic memory and restoration functions: After startup, all preset temperature data are automatically stored in the system;
Special black surface material characterized by its strong resistance to abrasion and corrosion.
Temp. Range
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RTto90°C
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Temp. Precision
|
1°C
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Over-Temp. Alarm Range
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1~5°C
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Environmental Temp. Range
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0~40°C
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Bath Tank Size(W*D*H)
|
240*180*55mm
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Display
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LCD
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Power Supply
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AC220V±10%» 50/60HZ; AC110V±10%, 60Hz
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Consumption
|
200W
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External Size(W*D*H)
|
345*340*130mm
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Package Size(W*D*H)
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440*440*220mm
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Gross Weight
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5kg
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Features
1.Integrating spreading, baking and roasting slices in one, compact structure (small space occupation), novel shape and easy operation;
2.The film drying box can dry the film at a constant temperature and can accommodate 3 dyeing racks;
3.The louver-style grilling table top has a large capacity (60 slides are actually arranged), easy to put and take out, and the grilling effect is better;
4.High-brightness LCD display, real-time display of sheet spreading and drying sheet temperature, simple and clear;
5.Highly sensitive temperature control integrated unit, high precision and reliable performance;
6.With over-temperature alarm function;
7.Using new heating software, fast heating, uniform heating, small thermal inertia, reliable energy saving;
8.With memory function, automatically retain the set temperature after operation;
9.The work surface is spray-coated, corrosion-resistant.
Tissue Flotation Water Bath & Slide Dryer
Technical Parameters:
Model |
BTH-I |
Temp. Range |
RTto 90°C |
Temp. Precision |
2°C |
Environmental Temp. Range |
0~40°C |
Over-Temp. Alarm Range |
1~5°C |
Drying Area |
344*260mm |
Bath Tank Size(W*D*H) |
304*207*52mm |
Display |
LCD |
Power Supply |
AC220V±10%, 50/60HZ; AC110V±10%» 60Hz |
Consumption |
600W |
External Size(W*D*H) |
695*400*21 Omm |
Package Size(W*D*H) |
800*510*320mm |
Gross Weight |
20kg |
1.Temperature range of water bath: RT - 70°C/RT - 100°C; automatically maintained
2.Automatic memory and restoration functions: After startup, all preset temperature data are automatically stored in the system.
3.Temperature Control Precision:±2°C
4.Dimensions of water bath dish: 210×170×65mm(W×D×H)
5.Dimensions of drying station: 250×108mm(W×D×H)
6.Working Voltage: AC 220V±10% 50Hz (standard model); 7.AC110V±10% 60Hz Power: 400W
8.Dimensions: 310×460×135 mm (W×D×H)
9.Net weight: 6kg
Features:
*Integrate tissue flotation water bath and slide dryer, compact structure and easy operation
*High-brightness LCD display, real-time display of temperature.
*Highly sensitive temperature control integrated unit, high precision and reliable performance.
*With over-temperature alarm function
*Using new heating materials, heating fast, uniform heating, low thermal inertia, energy saving and reliable.
*With memory function. The set temperature is automatically retained.
*Louver-type drying table, easy to operate, better drying effect
*The work surface is sprayed for corrosion resistance.
Laboratory Educational Single Concave Microscope Blank Glass Slides for Medical
Name |
Specification |
Corner |
Package |
7101 |
Plain, ground edges |
90°/45° |
50pcs/box, 50boxes/ctn, Measure: 36x18x16cm, G.W./N.W.:14/13KGS
72pcs/box, 50boxes/ctn, Measure: 44x20x16cm, G.W./N.W.: 20/19KGS |
7102 |
Plain, unground edges |
90°/45° |
7103 |
Singe concave, ground edges |
45° |
7104 |
Double concaves, ground edges |
45° |
7105 |
Single frosted end, ground edges |
90°/45° |
7105-1 |
Single frosted end, unground edges |
90° |
7106 |
Frosted both ends on one side, ground edges |
90° |
7107 |
Double frosted ends, ground edges |
90°/45° |
7107-1 |
Double frosted ends, unground edges |
90° |
7108 |
Frosted both ends on both sides, ground edges |
90° |
7109 |
Colour frosted end, ground edge |
90°/45° |
7110 |
Fully frosted on one side, ground edges |
90° |
7111 |
Poly-L-lysine Coated slide |
90°/45° |
7112 |
Positive charged slide |
90°/45° |
7113 |
Silane coated slide |
90°/45° |
Histology Tissue Slide Warmer Pathology Tissue Floating Water Bath For Glass Slide
Laboratory microscope slide clips frosted glass slide
Feature
1) 7101: Ground edges
2) 7102: Un-ground edges
3) 7103: Single concave, ground edges
4) 7104: Double concave, ground edges
5) 7105: Frosted one end on one side, ground edges
6) 7105-1: Frosted one end on one side, un-ground edges
7) 7106: Frosted both ends on one side, ground edges
8) 7107: Frosted one end on both sides, ground edges
Histology Tissue Slide Warmer Pathology Tissue Floating Water Bath For Glass Slide
The floating bath and slide warmer machine s
upports multiple baking modes of oblique insertion and staining rack, and the heating time can be set by oneself.Adopting high-definition color touch screen, the operation is simple and easy to understand, and it is easy to get started quickly.
The floating bath and slide warmer machine supports multiple baking modes of oblique insertion and staining rack, and the heating time can be set by oneself.
Adopting high-definition color touch screen, the operation is simple and easy to understand, and it is easy to get started quickly.
The slide warmer part has an oblique insertion baking mode makes it easier to pick up the glass slides
Capacity: 60 pcs slides
The tissue floating bath and slide warmer machine temperature range :0 to 99ºC
It's made of special materials,corrosion-resistant.