PCR Machine Amplification Gradient Thermal Cycle DNA/Rna Real Time PCR for Laboratory

• Customization: Available
• Capacity: 98 Well
• Channels: 6 Channels Fluorescence Detection
• Model NO.: PCR Gradient Thermal Cycler
• Prevent Reagent Evaporation: Yes
• Fluorescence: Yes
• Sample Test Repeatability: CV<3%
• Fluorescence Linearity: R≧0.99
• Transport Package: Carton Box
• Specification: 38*38*26
• Trademark: SAH
• Origin: China
• Production Capacity: 5000000

Product Description

Lab Fast Testing PCR Gradient Thermal Cycler Real-time Pcr Thermocycler System Clinical Analytical Machines Real Time Pcr
Real Time 48-well 96 Well Real time PCR Laboratory Thermal Cycler Gradient gene amplification instrument
Quantitative real time PCR detection system Machine
Whole block scanning and formulated line scanning mode
98 well double colour scanning takes only 5.5s.
6 channels fluorescence detection
No cross talk between different channels unique bottom detection compatible to reaction volume down to 5micro liter 
New automatic hot lid
Automatic open and close which is prevent reagent evaporation 
LED excitation light source with super long service life
 

Features

• Advanced optical system including full-spectrum high-power LED and high-sensitive PMT top scanning achieves higher sensitivity you need in researches and experiments.

• High-precision temperature control over the block with a resolution of 0.1 ºC offers reliable and consistent qPCR assay data.

• Gradient temperature range is 30-100ºC and the temperature difference between adjacent blocks can be high up to 40ºC for assay optimization.

• Sample tray automatically moves in/out, enabling integration with automated workstations/plate handlers for unmanned detection.

• 6 fluorescence channels without cross talk and 96-well high-throughput process at a single run meet a variety of qPCR assay demands. 

• The 10.1-inch large touch screen facilitates flexible programming and efficient running of PCR protocols.

• The intuitive built-in software and PC software for improved user's experience allow you to run and analyze qPCR data from personal computer

• Two temperature control modes enable you to control either block or tube temperatures.
   

   

Multiple Applications

The Real-Time Fluorescent Quantitative PCR System uses fluorescence real-time detection method to analyze the amplification of PCR template. It is suitable for human genome engineering, forensic medicine, oncology, population biology, paleontology, zoology, botany and other research fields.

This PCR detection system can be an in vitro diagnostic medical device, which is suitable for polymerase chain reaction fluorescence quantitative detection in the field of clinical diagnosis of viruses, tumors, genetic diseases, etc.

PCR machine is also known as thermal cyclers or DNA amplifiers, or thermocyclers. PCR machines amplify small segments of DNA or RNA chosen from the genome with a primer. It is inexpensive and a very effective tool.
Real-time PCR, also known as quantitative PCR or qPCR, is a technique for monitoring and quantifying PCR results in real-time by labeling DNA molecules with fluorescent dye.

PCR Applications
PCR can investigate differences in gene transcription among various cell types, tissues, and species at a particular time period.

• Reverse transcription is used to create cDNA by isolating RNA  from samples of interest.
• The quantity of cDNA produced by PCR can then be used to calculate the original RNA levels for a particular gene.
• Sequence differences in alleles of certain cells or organisms can be determined.
• Genotyping of transgenic organisms facilitates the amplification of the mutation or a transgenic part.
• PCR cloning makes it possible to breed new strains of bacteria with altered genetic makeup via the insertion of amplified dsDNA fragments into the vectors such as gDNA, cDNA, and plasmid DNA.
• Cloning assists in introducing point mutations using site-directed mutagenesis which itself employs the recombinant PCR method.
• It also helps to create novel gene fusions.
• Sequencing accompanies the amplification of template DNA, their purification, and processing through a sequencing step.
• PCR is also employed in next-generation sequencing (NGS) during the library preparation phase to quantify DNA samples and tag them with sequencing adaptors for multiplexing.
• Medical applications include genetic changes linked to disease to identifying infectious organisms. Prenatal genetic testing employs PCR to detect chromosomal abnormalities and genetic mutations in the pregnancy, providing expecting parents with crucial information about the likelihood that their children will have a particular genetic illness.
• It can also be employed as a preimplantation genetic diagnosis technique to screen embryos for in vitro fertilization (IVF).
• PCR can be useful for forensic investigations to identify the sources of samples and paternity testing.
• It is used in molecular archaeology to amplify DNA from artifacts.
• Pathogens can be found using PCR, not just in patient samples but also in matrices like food and water. This is important for both diagnosing and preventing infectious diseases.

The PCR Machine promotes polymerase chain reaction (PCR) allowing vitro amplification of DNA fragments as fast cloning, DNA sample modification, detecting pathogenic microorganisms and accurate genotyping, detection of mutation, malignancy transformation or tissue typing, analysis of the archaeological specimen, forensic applications, paternity testing, mapping hereditary traits, and investigating gene expression.  

PCR steps
PCR involves three main steps repeated in cycles:
1.Denaturation
The mixture is heated to 94°C for 0.5 to 2 minutes.
This heat breaks the hydrogen bonds between DNA strands, turning them into single strands.
The single strands serve as templates for creating new DNA strands.
2.Annealing
The temperature is lowered to 54-60°C for 20-40 seconds.
Primers, which are short DNA or RNA sequences, bind to the complementary sequences on the single-stranded DNA.
Each separated strand gets a forward and reverse primer.
3.Elongation
The temperature is raised to 72-80°C.
The taq polymerase enzyme adds DNA bases to the 3' end of the primer, extending the DNA strand in the 5' to 3' direction.
This enzyme can withstand high temperatures and works quickly, adding about 1000 bases per minute.
These three steps are repeated 20-40 times to rapidly amplify the target DNA sequences.

Specifications

Throughput	96 well (12X8)
Applicable consumables	PCR single tube and strip of 0.2 ml, 96-well half skirted / no skirted plate PCR single tube and strip of 0.1 ml, 96-well no skirted plate
Dynamic range	1-10^10copies
Detection location	Top detection
Excitation light wavelength	400-800nm
Detection wavelength	500-800nm
Fluorescence channels	6 channels
Fluorochrome / dye	Channel 1: FAM/SYBR Green I      Channel 2: JOE/HEX/TETA/IC Channel 3: NED/TAMRA/Cy3       Channel 4: ROX/Texas Red Channel 5: Cy5                             Channel 6: Cy5.5
Excitation light source	full-spectrum LED
Detector	PMT
Block temperature range	4-105°C
Block temperature accuracy	≤±0.1ºC
Block temperature precision	≤±0.1ºC
Block temperature uniformity	≤±0.1ºC
Max. heating & cooling rate of block	≥6ºC/S
Gradient temperature difference	1-40°C
Block temperature control mode	Block mode, Tube mode
Sample volume range	5-100 µL
Heated lid temperature range	30°C-110°C (default 105°C)
Fluorescence intensity	CV ≤3%
Detection repeatability	Yes
Touch screen	10.1-inch touch screen
Scan mode	Full plate scan or specified line scan
Software functions	Absolute quantification, Relative quantification, Melting curve, SNP genotyping, HRM, Quick run, etc.
Operation system	PC software (Windows 8 or higher), Built-in software
Ports	USB Type-A port x 2, USB Type-B port, RJ45 port
Power-off protection function	YES. Data can be restored after power-on
Sample tray control mode	Sample tray automatically moves in/out, enabling integration with automated workstations/plate handlers.
Dimension (WxDxH)	320mm x 525mm x 420mm
Net weight	≤23kg
Thermal Cycling Technology	Peltier Module
Memory	8GB

A real-time PCR system is a powerful laboratory instrument that enables researchers to monitor and quantify DNA or RNA amplification in real-time. This technology relies on the use of fluorescent markers that emit signals during each amplification cycle, allowing for the accurate determination of the starting amount of genetic material. It can also simultaneously detect and quantify multiple target sequences in a single reaction, saving time and resources. The high sensitivity, precision, and versatility of real-time PCR make it an essential tool across an array of scientific domains such as genetics research, clinical diagnostics, microbiology, and environmental monitoring. In practice, it furnishes researchers with an unparalleled opportunity to delve into the intricacies of gene expression, diagnose diseases, and undertake a broad spectrum of molecular biology investigations with unwavering accuracy and operational efficiency.

 

 

7.CNC machining for the benchtop Microtome machine

The rotor was the key part for the Universal Digital Thermostatic Ultrasonic Automatic Rotary Microtome ,to make it perfect especial for the Universal Digital Ultrasonic Automatic Rotary Microtome Equipment ,we need to control the tolerance of the rotor very tight,that was why we introduce the High Temperature Automatic Rotary Microtome
1.CNC QTY:15PCS
2.Tolerance for CNC machine :0.01mm

8.Universal Digital Ultrasonic Semi Automatic Microtome stamping ,welding ,rolling Production process on site,the container of the Ultrasonic Semi Automatic Microtome Machine need the vacuum and seal can bear though high pressure 

9.Electric part assemble to the  Multifunction Steam Sterilization Machine workshop,the plc or the other computer control part connect to the machine before move to the next production procss.

10.Test workshop for the  Multifunction Steam Sterilization Machine  

All  Multifunction Steam Sterilization Machine need to be test before transfer it to the warehouse.

Customer visit

Customer review our Fully Automated Tissue Steiner  workshop,We were welcome all of our customer to visit our company to review and test the Fully Automated Tissue Steiner for Organisms, Medical, Chemistry,Our business vision was :long term business ,win-win for the future

Packing and loading

The over sea customer always concern about the packing and loading for the Fully Automated Tissue Steiner Machine,we cant bear the lost for tiny mistake for the Vacuum Steam Machine machine ,one mistake will cause the machine broken or cant work .
From the packing for Laboratory Medical Sterilization Machine to the container loading detail show as below 

Warehouse and stroage for the medical Medical Sterilization Machine,the temperature should be control within 18-25° for all equipment and test machine ,all the record can trace within 3 years.
 

 

After market service

The maintain and repair guarantee for the Medical Sterilization Machine workshop was the key to the business ,when a customer bought a machine from us ,the guarantee was the most importance things customer concern.we sent out technical service team to oversea customer site for the maintain and repair for our customer .Our service for the lab equipment was not stop after the Vacuum Steam Machine Machine sold ,it was the start for our company and your company for win win situation .You use the Vacuum Steam Machine Machine we take care of the rest.

Exhibition

We take part into the MedtecChina Canton Fair (The China Import and Export Fair) exhibition every year ,hope one day we can meet each on the fair

Our vision

The supplier and the customer was togeher for the future ,both parties should be in win-win situation ,so we were looking forwarder to hold hand by hand for bright future.